Heavy water and15N labelling with NanoSIMS analysis reveals growth rate-dependent metabolic heterogeneity in chemostats
نویسندگان
چکیده
منابع مشابه
Heavy water and (15) N labelling with NanoSIMS analysis reveals growth rate-dependent metabolic heterogeneity in chemostats.
To measure single-cell microbial activity and substrate utilization patterns in environmental systems, we employ a new technique using stable isotope labelling of microbial populations with heavy water (a passive tracer) and (15) N ammonium in combination with multi-isotope imaging mass spectrometry. We demonstrate simultaneous NanoSIMS analysis of hydrogen, carbon and nitrogen at high spatial ...
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ژورنال
عنوان ژورنال: Environmental Microbiology
سال: 2015
ISSN: 1462-2912
DOI: 10.1111/1462-2920.12752